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We showed that cooperative binding of TFs to DNA is a common phenomenon at active enhancers. And, the distances between bound TFs suggest nucleosome-mediated cooperativity, meaning that the TFs bind synergistically to evict nucleosomes and keep enhancers open for their function. Dual Enzyme Single Molecule Footprinting (dSMF)data played a key role in addressing the problem. Briefly, CpG and GpC methyltransferases are used to methylate accessible cytosines on chromatinized DNA. Thus, a DNA-bound protein leaves a footprint that can be captured on individual sequenced DNA molecules. Please read the article here and its coverage in the Preview.